Template Switch Oligo

Template Switch Oligo - (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso with rgrgrg at the 3′ end. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by pcr or serve as. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers.

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end. The resulting cdna can be amplified by pcr or serve as. However, if a large fraction of the library contains a portion. However, if a large fraction of the library contains the. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Use this set of oligonucleotides for template switching cdna synthesis and preamplification.

(A) The TS mechanism is used for first strand cDNA synthesis. First, an

A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains the. In conjunction with a template switching oligo.

Template switching oligos (TS oligos, TSOs) for cDNA library

The resulting cdna can be amplified by pcr or serve as. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of visium libraries.

Template switch and twin priming. (A) Steps describing template

Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In general, different tsos.

Tuning 5’ to internal read proportions and template switching oligo PCR

(step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by pcr or serve as. Template switching.

Fig. S12 Schematic representation of the tested oligodT / TSO

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of single cell.

NanoCAGE A HighResolution Technique to Discover and Interrogate Cell

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains a portion. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend.

(A) First strand cDNA is initiated by priming with an oligo dT primer

We recommend a tso with rgrgrg at the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Template switching oligonucleotide and capturing oligo (dt) are used.

Template Switch Oligo

A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Template switching oligonucleotide and capturing oligo (dt).

Template Switch Oligo

However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of single cell 3' libraries are expected to contain the template switching.

Template Switching Oligo

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The resulting cdna can be amplified by pcr or serve as. (step 2) mmlv reverse.

The Resulting Cdna Can Be Amplified By Pcr Or Serve As.

In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. We recommend a tso with rgrgrg at the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end.

In Conjunction With A Template Switching Oligo (Tso), Cdna Is Synthesized With A Known Sequence Of Choice Attached To The 3′ End.

However, if a large fraction of the library contains the. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Use this set of oligonucleotides for template switching cdna synthesis and preamplification.